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Bioengineering

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JoVE Journal Bioengineering
I Vitro Modell av Human Kutan Hypertrofisk Arrdannelse ved hjelp av makromolekylær trengsel
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I Vitro Modell av Human Kutan Hypertrofisk Arrdannelse ved hjelp av makromolekylær trengsel

Article DOI: 10.3791/61037-v • 08:20 min • May 1st, 2020
May 1st, 2020 •
1, 1, 1, 1, 1,2, 1, 1,3, 1
1Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences, Singapore Polytechnic, 3Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)

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Denne protokollen beskriver bruken av makromolekylær trengsel for å skape en in vitro menneskelig hypertrofisk arrvevsmodell som ligner på vivo-forhold. Når dyrket i en overfylt makromolekylærmiljø, menneskelig hud fibroblaster viser fenotyper, biokjemi, fysiologi, og funksjonelle egenskaper som ligner arrvev.

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Bioengineering Utgave 159 hypertrofisk arr fibroblaster makromolekylær trengsel kollagen ekstracellulær matrise tetthet gradient medium polyvinylpyrrolidon
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