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Journal / Immunology and Infection / P. aeruginosa Infected 3D Co-Culture of Bronchial …
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Immunology and Infection

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JoVE Journal Immunology and Infection
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
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P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

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Summary
Automatic Translation

We describe a protocol for a three-dimensional co-culture model of infected airways, using CFBE41o- cells, THP-1 macrophages, and Pseudomonas aeruginosa, established at the air-liquid interface. This model provides a new platform to simultaneously test antibiotic efficacy, epithelial barrier function, and inflammatory markers.

Tags

P. Aeruginosa Infected 3D Co-culture Bronchial Epithelial Cells Macrophages Air-liquid Interface Preclinical Evaluation Anti-infectives Bacteria Biofilms Host Cells Epithelial Host Cells Biological Barriers Monological Response Novel Anti-infectives Organ Affected Lung Drug Administration Aerosol Human Cells Species Differences Infection Research Molecular Events Cellular Events
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