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Journal / Immunology and Infection / P. aeruginosa Anti-Enfektiflerin Klinik Öncesi…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
P. aeruginosa Anti-Enfektiflerin Klinik Öncesi Değerlendirilmesi Için Hava-Sıvı Arabiriminde Bronşiyal Epitel Hücrelerin in enfekte 3D Eş Kültür
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Journal (Immunology and Infection)
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Journal (Immunology and Infection)
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
 
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P. aeruginosa Anti-Enfektiflerin Klinik Öncesi Değerlendirilmesi Için Hava-Sıvı Arabiriminde Bronşiyal Epitel Hücrelerin in enfekte 3D Eş Kültür

Article DOI: 10.3791/61069-v • 10:26 min • June 15th, 2020
June 15th, 2020 •
*1,2, *1,2, 3, 4, 4, 3, 1, 1, 1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
* These authors contributed equally

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Biz cfbe41o kullanarak enfekte hava yollarının üç boyutlu ortak kültür modeli için bir protokol tarif- hücreler, THP-1 makrofajlar, ve Pseudomonas aeruginosa, hava-sıvı arayüzü nde kurulan. Bu model aynı anda antibiyotik etkinliğini test etmek için yeni bir platform sağlar, epitel bariyer fonksiyonu, ve inflamatuar belirteçleri.

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