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Journal
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Immunology and Infection
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使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和
+
排水进行三维成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO
+
and Light Sheet Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和
+
排水进行三维成像
DOI:
10.3791/61099-v
•
10:05 min
•
May 22, 2020
•
Laurent Jacob*
1
,
Jose Brito*
1,2
,
Jean-Leon Thomas
3
1
Université Pierre et Marie Curie Paris, Sorbonne Université
,
Institut du Cerveau et de la Moelle Epinière
,
2
Instituto de Ciências Biomédicas
,
Universidade Federal do Rio de Janeiro
,
3
Department of Neurology
,
Yale University School of Medicine
Chapters
00:04
Introduction
00:49
Intra-Cisterna Magna (ICM) and Thoracolumbar (ThLb) Injection
02:22
Perfusion and Tissue Dissection
03:34
Whole Mount Vertebral Segment Immunolabeling-Enabled Imaging of Solvent-Cleared Organs-Plus (iDISCO
+
)
06:14
Light Sheet Fluorescence Microscopy (LSFM) Imaging
08:02
Results: Representative Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage
09:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种将组织清除与光片荧光显微镜 (LSFM) 相结合的协议,以获得收集脑脊液 (CSF) 和脊柱硬膜外液的淋巴血管和淋巴结 (LNs) 的三维和细胞分辨率图像。
Tags
3D Imaging
Vertebral Lymphatic Vasculature
Lymphatic Drainage
IDISCO+ Clearing
Light Sheet Fluorescence Microscopy
Spinal Cord
Meninges
Epidural Space
Draining Lymph Nodes
Fluorescent Tracer
Perfusion
Anatomical Imaging
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