Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolamento delle cellule endocartali e coronarie…
JoVE Journal
Developmental Biology

This content is Open Access.

JoVE Journal Developmental Biology
Isolamento delle cellule endocartali e coronarie dal muro libero ventricolare del cuore di ratto
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
Journal (Developmental Biology)
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
Journal (Developmental Biology)
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
 
Click here for the English version

Isolamento delle cellule endocartali e coronarie dal muro libero ventricolare del cuore di ratto

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi presentiamo un protocollo per l'isolamento delle cellule endocardiali e coronariche dai cuori dei ratti attraverso la digestione sequenziale dei tessuti in un buffer di digestione, la raccolta cellulare da cicli di centrifuga ricorrenti e la purificazione cellulare utilizzando microsfere CD31 anti-rat.

Tags

Biologia dello sviluppo numero 158 endocardio endotelio coronario isolamento cuore ratto CD31
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter