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Journal / Developmental Biology / Isolatie van endocardiale en coronaire…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Isolatie van endocardiale en coronaire endotheelcellen van de Ventriculaire Vrije Muur van het Hart van de Rat
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Isolatie van endocardiale en coronaire endotheelcellen van de Ventriculaire Vrije Muur van het Hart van de Rat

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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We presenteren een protocol voor de isolatie van endocardiale en coronaire endotheelcellen uit rattenharten door sequentiële weefselvertering in een spijsverteringsbuffer, celverzameling uit terugkerende centrifugecycli en celzuivering met behulp van antiratten-CD31 microbeads.

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Ontwikkelingsbiologie nummer 158 endocardium coronaire endotheel isolatie hart rat CD31
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