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JoVE Journal
Biology

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Massespektrometrianalyse for å identifisere Ubiquitylation av EYFP-merket CENP-A (EYFP-CENP-A)
 
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Massespektrometrianalyse for å identifisere Ubiquitylation av EYFP-merket CENP-A (EYFP-CENP-A)

Article DOI: 10.3791/61138-v 09:02 min June 10th, 2020
June 10th, 2020

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Summary

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Click here for the English version.

CENP-A ubiquitylation er et viktig krav for CENP-A deponering ved centromere, arvet gjennom dimerisering mellom celledeling, og uunnværlig for celle levedyktighet. Her beskriver vi massespektrometrianalyse for å identifisere ubiquitylation av EYFP-merket CENP-A (EYFP-CENP-A) protein.

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Biologi utgave 160 centromere kinetokore mitose CENP-A etter oversettelsesmodifisering PTM ubiquitylation massespektrometrianalyse
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