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Massespektrometrianalyse for å identifisere Ubiquitylation av EYFP-merket CENP-A (EYFP-CENP-A)
JoVE Journal
Biology
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JoVE Journal Biology
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
DOI:

09:02 min

June 10, 2020

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Chapters

  • 00:04Introduction
  • 00:44In Vivo Ubiquitylation Assays Using pQCXIP-EYFP-CENP-A
  • 02:19Mass Spectrometry to Identify the Ubiquitylation Site of the EYFP-CENP-A K124R Mutant
  • 04:55Data Analysis
  • 06:22Results: EYFP-CENP-A K124 Mutant is Ubiquitylated and Interacts with HJURP
  • 08:12Conclusion

Summary

Automatic Translation

CENP-A ubiquitylation er et viktig krav for CENP-A deponering ved centromere, arvet gjennom dimerisering mellom celledeling, og uunnværlig for celle levedyktighet. Her beskriver vi massespektrometrianalyse for å identifisere ubiquitylation av EYFP-merket CENP-A (EYFP-CENP-A) protein.

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