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JoVE Journal
Biology

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Massaspectrometrieanalyse om alomtegenwoordigheid van EYFP-tagged CENP-A (EYFP-CENP-A) te identificeren
 
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Massaspectrometrieanalyse om alomtegenwoordigheid van EYFP-tagged CENP-A (EYFP-CENP-A) te identificeren

Article DOI: 10.3791/61138-v 09:02 min June 10th, 2020
June 10th, 2020

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Summary

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CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Hier beschrijven we massaspectrometrieanalyse om alomtegenwoordigheid van EYFP-tagged CENP-A (EYFP-CENP-A) eiwit te identificeren.

Tags

Biologie centromere kinetochore mitose CENP-A post-translationele modificatie PTM alomtegenwoordigheid massaspectrometrie analyse
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