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乳腺癌临床前模型乳腺肿瘤的矫形移植

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Cancer Research

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JoVE Journal Cancer Research

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

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乳腺癌临床前模型乳腺肿瘤的矫形移植

DOI:

10.3791/61173-v

•

07:45 min

•

May 18, 2020

•

Xiangdong Lv*^1,2,3, Lacey E. Dobrolecki*^1,2,3, Yao Ding^2,3, Jeffrey M. Rosen^2,3, Michael T. Lewis^2,3, Xi Chen^2,3

¹Department of Molecular and Cellular Biology,Baylor College of Medicine, ²Lester and Sue Smith Breast Center,Baylor College of Medicine, ³Dan L. Duncan Cancer Center,Baylor College of Medicine

Chapters

00:04Introduction
01:22Cryopreserved Mammary Tumor Fragment Preparation
02:23Fresh Mammary Tumor Sample Collection and Preparation
03:28Surgical Preparation
04:04Tumor Fragment Transplantation
06:26Results: Representative Transplant Tumor Characterization
06:54Conclusion

Summary

Automatic Translation

患者衍生异种移植（PDX）模型和可移植的基因工程小鼠模型忠实地概括了人类疾病，是基础和转化乳腺癌研究的首选模型。在这里，一种方法被描述为正位移植乳房肿瘤片段到乳腺脂肪垫研究肿瘤生物学和评估药物反应。

Tags

Orthotopic Transplantation Breast Tumor Preclinical Model Breast Cancer Tumor Biology Drug Response Drug Resistance Mammary Fat Pad Patient-derived Xenograft Subcutaneous Implantation Cryopreservation Thawing Tumor Dissection DMEM

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