Journal
/
Cancer Research
/
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

DOI:
10.3791/60622-v

•

06:16 min

•

December 07, 2019

•

, ,
1Division of Cancer, Department of Surgery and Cancer,Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute,Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

Chapters

  • 00:04Title
  • 00:37Digestion of Pancreatic Tumors
  • 01:24Preparation of Single-cell Suspension from Digested Tumor
  • 03:04Preparing Cells for Ex Vivo Stimulation
  • 03:57Results: Flow Cytometry Analysis and Quantification of Ex Vivo Spleen- and Tumor-derived T Cell Activity
  • 05:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the surgical generation of orthotopic pancreatic tumors and the rapid digestion of freshly isolated murine pancreatic tumors. Following digestion, viable immune cell populations can be used for further downstream analysis, including ex vivo stimulation of T cells for intracellular cytokine detection by flow cytometry.

Tags

Keywords: Orthotopic Pancreatic TumorsTumor-infiltrating T CellsCytotoxicityTumor DigestionEx Vivo CharacterizationTherapeutic AgentsT Cell ResponseFlow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60622?article_type=v

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
check_url/60622?article_type=v

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
check_url/60622?article_type=v

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
check_url/60622?article_type=v

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
check_url/60622?article_type=v

Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models
check_url/60622?article_type=v

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
check_url/60622?article_type=v

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
check_url/60622?article_type=v

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
check_url/60622?article_type=v

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
check_url/60622?article_type=v

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Read Article