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JoVE Journal
Cancer Research

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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
 

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Article DOI: 10.3791/60022-v 07:01 min
March 1st, 2020

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Summary March 1st, 2020

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.

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