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JoVE Journal
Cancer Research

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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
 

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Article doi: 10.3791/60022
March 1st, 2020

Summary March 1st, 2020

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.

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