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Journal / Genetics / Methyleret RNA Immunoprecipitation Assay at…
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Genetics

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JoVE Journal Genetics
Methyleret RNA Immunoprecipitation Assay at studere m5C Modifikation i Arabidopsis
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Methyleret RNA Immunoprecipitation Assay at studere m5C Modifikation i Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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Den methylerede RNA-immunoprecipitationsanalyse er en antistofbaseret metode, der bruges til at berige methylerede RNA-fragmenter. Kombineret med dyb sekventering fører det til identifikation af udskrifter, der bærer m5C-modifikationen.

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Genetik Problem 159 RNA-methylering 5-methylcytosin (m5C) immunoprecipitation (IP) Arabidopsis epitranscriptomics mRNA RNA-sekventering
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