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JoVE Journal Bioengineering
Automatisierung von Bio-Rasterkraftmikroskop-Messungen an Hunderten von C. albicans-Zellen
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Automatisierung von Bio-Rasterkraftmikroskop-Messungen an Hunderten von C. albicans-Zellen

Article DOI: 10.3791/61315-v • 09:27 min • April 2nd, 2021
April 2nd, 2021 •
*1, *1,2,3, 4, 3,5, 2
1ITAV-CNRS, Université de Toulouse, CNRS, Toulouse, France, 2LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France, 3ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico, 4TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France, 5CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico
* These authors contributed equally

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Dieses Protokoll zielt darauf ab, AFM-Messungen an Hunderten von mikrobiellen Zellen zu automatisieren. Zuerst werden Mikroben in PDMS-Stempelmikrostrukturen immobilisiert und dann werden kraftspektroskopische Messungen automatisch an Hunderten von immobilisierten Zellen durchgeführt.

Tags

Rasterkraftmikroskopie C. albicans Automatisierung Zellnanomechanik Mechanobiologie Kraftkurvenanalyse Zelladhäsion AFM-Automatisierung Jython JPK-Experimentplaner
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