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A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
DOI:

12:18 min

August 08, 2020

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Chapters

  • 00:00Introduction
  • 02:34The Setting Up of the Thermal Injury Device
  • 03:09Multimodal Analgesia and Fluid Replacement
  • 04:25Anaesthesia and Maintenance of Corneal Hydration
  • 05:07Full Thickness Burn Wound Induction with Heated Brass Block
  • 06:43Surgical Excision of Burned Tissue, Preparation of the Graft Recipient Site and Skin Grafting Procedure
  • 09:10Results: Macroscopic and Microscopic Apperance of Grafted Burn Wounds
  • 11:31Conclusion

Summary

Automatic Translation

The aim of this study was to develop a murine model of burn wound healing. A thermal burn was induced on the dorsal skin of mice using a preheated brass template. Burned tissue was debrided and overlaid with a skin graft harvested from the tail of a genetically similar donor mouse.

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