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Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
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Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

English

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1,603 Views

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10:03 min

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November 18, 2022

DOI:

10.3791/64836-v

DOI:
10.3791/64836-v

•

10:03 min
November 18, 2022

•

4 Views
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1Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center,Harvard Medical School, 2Section for Immunology, Joslin Diabetes Center,Harvard Medical School

Summary

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This protocol describes a straightforward and minimally invasive method for transplanting and imaging NIT-1 cells in non-obese diabetic (NOD)-severe combined immunodeficient mice challenged with splenocytes purified from spontaneously diabetic NOD mice.

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Bioluminescent MonitoringGraft SurvivalAdoptive TransferAutoimmune DiabetesMiceCell CultureTransplantImagingTail Vein InjectionIsofluraneSubcutaneous InjectionCell Suspension

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Cite this Article

Stewart, T., Bode, K., Kissler, S., Yi, P. Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice. J. Vis. Exp. (189), e64836, doi:10.3791/64836 (2022).

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