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Journal / Biology / 应用激光微分射发现人体肾脏组织中的区域转录学…
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应用激光微分射发现人体肾脏组织中的区域转录学
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应用激光微分射发现人体肾脏组织中的区域转录学

Article DOI: 10.3791/61371-v • 05:46 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 3, 4, 1, 1, 1, For The Kidney Precision Medicine Project,
1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine

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我们描述了人类肾脏的子段的激光微分射方案，包括球状、近肌管、厚升肢、收集管道和间质。然后从获得的隔间中分离RNA，进行RNA测序，以确定每个子段内转录签名的变化。

Tags

生物学， 第 160 期， 激光微分， LMD， 协议， 肾脏， 球菌， 管子段
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