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Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue
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JoVE Journal Biology
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue

DOI:
10.3791/61371-v

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05:46 min

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June 09, 2020

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, , , , , , , , , , , , ,
1Department of Medicine,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine,Ohio State University Wexner Medical Center, 4Division of Pathology,Indiana University School of Medicine

Chapters

  • 00:05Introduction
  • 00:41Cryosectioning and Laser Microdissection (LMD)
  • 03:39Results: Representative Human Kidney Regional Transcriptomics Analyses
  • 05:05Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en protokol for laser mikrodissementering af undersegmenter af den menneskelige nyre, herunder glomerulus, proksimal tubule, tykt stigende lem, opsamlingskanal og interstitium. RNA'et isoleres derefter fra de opnåede rum, og der udføres RNA-sekventering for at bestemme ændringer i transskriptionssignaturen inden for hvert undersegment.

Tags

Laser MicrodissectionTranscriptomicsRegional AnalysisSingle Cell RNA SequencingCryosectioningImmunofluorescenceLaser Microdissection PlatformRNA IsolationLowly Expressed Transcripts

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