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Anvendelse av lasermikrodisseksjon for å avdekke regionale transkripsjonsmikromikk i humant nyrevev
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Anvendelse av lasermikrodisseksjon for å avdekke regionale transkripsjonsmikromikk i humant nyrevev

Article DOI: 10.3791/61371-v • 05:46 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 3, 4, 1, 1, 1, For The Kidney Precision Medicine Project,
1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine

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Vi beskriver en protokoll for lasermikrodisseksjon av undersegmenter av den menneskelige nyren, inkludert glomerulus, proksimal tubule, tykk stigende lem, samlekanal og interstitium. RNA blir deretter isolert fra de oppnådde rommene, og RNA-sekvensering utføres for å bestemme endringer i transkripsjonssignaturen i hvert undersegment.

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Biologi Utgave 160 Lasermikrodisseksjon LMD protokoll nyre glomerulus rørformet undersegment
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