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Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
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Cancer Research
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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

DOI:
10.3791/61399-v

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05:18 min

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June 09, 2020

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, , , ,
1Nuclear Facilities Operations Department,National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

Chapters

  • 00:04Introduction
  • 00:54Fixation and Permeabilization of Cells
  • 01:53Immunofluorescence Staining
  • 03:16Results: Patterns of -H2AX, DNA-PKcs, and Rad52 Foci in Colon Cancer Cells
  • 04:24Conclusion

Summary

Automatic Translation

Automatic Translation

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

Tags

ImmunofluorescenceDNA DamageDNA RepairColon Cancer CellsNeutron-mixed BeamBNCTProton Beam TherapyHadron TherapyRadiation-induced FociRepair ProteinsGamma-H2AX Foci

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