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Cancer Research

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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
 

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Article doi: 10.3791/61399
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

Summary
Automatic Translation
June 9th, 2020

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

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