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Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

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05:18 min

June 09, 2020

DOI:

05:18 min
June 09, 2020

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Summary

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Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

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