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June 09, 2020
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Summary
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Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.
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Cite this Article
Maliszewska-Olejniczak, K., Dróżdż, A., Waluś, M., Dorosz, M., Gryziński, M. A. Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells. J. Vis. Exp. (160), e61399, doi:10.3791/61399 (2020).
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