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人类结肠癌细胞DNA损伤与修复福西的免疫荧光成像
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
DOI:

05:18 min

June 09, 2020

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Chapters

  • 00:04Introduction
  • 00:54Fixation and Permeabilization of Cells
  • 01:53Immunofluorescence Staining
  • 03:16Results: Patterns of -H2AX, DNA-PKcs, and Rad52 Foci in Colon Cancer Cells
  • 04:24Conclusion

Summary

Automatic Translation

在中子捕获治疗(BNCT)中使用的中子混合束激活的辐射诱导DNA损伤反应尚未完全确定。该协议提供了一个分步程序,通过使用中子混合束照射后,通过人体结肠癌细胞系中的免疫荧光染色来检测修复蛋白质的辐射诱导 foci (RIF)。

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