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Immunofluorescens Imaging af DNA-skader og reparation Foci i Human Colon Cancer Cells

Article DOI: 10.3791/61399-v • 05:18 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1, 1
1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

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Strålingsinduceret DNA-skadesrespons, der aktiveres af neutronblandet stråle, og som anvendes til behandling med neutronfangsning (BNCT), er ikke fuldt ud klarlagt. Denne protokol giver en trin-for-trin procedure til påvisning af stråling-induceret foci (RIF) af reparation proteiner ved immunfluorescens farvning i human tyktarmskræft cellelinjer efter bestråling med neutron-blandet stråle.

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Cancer Research BNCT DNA-DSBs stråling-induceret foci DNA-skader reparation veje blandet stråle
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