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Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space
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1Department of Medicine,University of Virginia, 2Department of Cell Biology,SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program,SUNY Downstate Medical Center, 4Division of Neonatology,University of Virginia, 5Department of Neuroscience and Physiology,New York University School of Medicine
Chapters
- 00:05Title
- 01:01Real-time Iontophoresis in Agarose
- 03:59Agarose Data Analysis
- 05:30Real-time Iontophoresis in Brain Slices
- 07:02Brain Data Analysis, Checking Transport Number and ISM Calibration
- 08:19Results: Curve Fitting Data in Agar and in Brain
- 09:21Conclusion
This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.
