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Måling naturlig ervervet phagocytosis-induserende antistoffer mot Plasmodium falciparum parasitter ved en Flow Cytometry-basert analyse
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1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences,University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology,University of Ghana, 3Department of Immunology,Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases,Rigshospitalet
Chapters
- 00:04Introduction
- 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
- 01:58Parasite Staining and Opsonization Plate Preparation
- 03:47THP-1 Cell and Phagocytosis Plate Preparation
- 04:35Phagocytosis Assay
- 06:21Flow Cytometry Acquisition and Analysis
- 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
- 09:21Conclusion
Det overordnede målet med denne protokollen er å gi instruksjon om hvordan man måler kapasiteten til antistoffer som finnes i sera eller plasma av individer, naturlig utsatt for Plasmodium falciparum infeksjon, å opsonisere og indusere fagocytose av parasitt-infiserte erytrocytter (IE).
