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Medindo anticorpos indutores de fagocitose naturalmente adquiridos para parasitas plasmodium falciparum por um ensaio baseado em citometria de fluxo
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay
DOI:

09:57 min

August 06, 2020

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Chapters

  • 00:04Introduction
  • 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
  • 01:58Parasite Staining and Opsonization Plate Preparation
  • 03:47THP-1 Cell and Phagocytosis Plate Preparation
  • 04:35Phagocytosis Assay
  • 06:21Flow Cytometry Acquisition and Analysis
  • 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
  • 09:21Conclusion

Summary

Automatic Translation

O objetivo geral deste protocolo é fornecer instruções sobre como medir a capacidade dos anticorpos presentes no soro ou plasma de indivíduos, naturalmente expostos à infecção por Plasmodium falciparum, para opsonizar e induzir fagocitose dos eritrócitos infectados por parasitas (IEs).

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