Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Aislamiento de núcleos de tumores cerebrales…
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.

JoVE Journal Cancer Research
Aislamiento de núcleos de tumores cerebrales frescos congelados para RNA-seq de un solo núcleo y ATAC-seq
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
Journal (Cancer Research)
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
Journal (Cancer Research)
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
 
Click here for the English version

Aislamiento de núcleos de tumores cerebrales frescos congelados para RNA-seq de un solo núcleo y ATAC-seq

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

La heterogeneidad intramoral es una característica inherente de los tumores, incluidos los gliomas. Desarrollamos un protocolo simple y eficiente que utiliza una combinación de tampones y centrifugación por gradiente para aislar núcleos individuales de tejidos de glioma frescos congelados para estudios de secuenciación de ARN de núcleo único y ATAC.

Tags

Investigación del cáncer Número 162 Aislamiento de núcleos snRNA-seq snATAC-seq Genómica 10x Glioma
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter