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利用单分子定位显微镜分析人类 iPSC 衍生肌细胞中的α-Actinin 网络
JoVE Journal
Biology
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JoVE Journal Biology
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
DOI:

07:02 min

November 03, 2020

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Chapters

  • 00:04Introduction
  • 00:27Photoactivated Localization Microscopy (PALM) Image Acquisition Setup
  • 01:27PALM Image Acquisition
  • 01:50PALM Data Reconstruction
  • 02:39Reconstructed PALM Image Post Processing
  • 03:34Sarcomere Filament Analysis
  • 04:41Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis
  • 06:23Conclusion

Summary

Automatic Translation

形成适当的沙康网络对于 iPSC 衍生心肌细胞的成熟非常重要。我们提出了一种基于超分辨率的方法,允许对干细胞衍生心肌细胞的结构成熟进行定量评估,以改善促进心脏发育的培养条件。

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