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Journal
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Developmental Biology
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牛早期蚁卵泡卵母细胞的体外培养策略
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Please note that all translations are automatically generated.
Click here for the English version.
牛早期蚁卵泡卵母细胞的体外培养策略
DOI:
10.3791/61625-v
•
09:30 min
•
July 08, 2020
•
Rodrigo G. Barros
,
Valentina Lodde
,
Federica Franciosi
,
Alberto M. Luciano
1
Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety
,
University of Milan
Chapters
00:04
Introduction
00:50
Media Preparation
01:44
Ovary Collection and Processing
03:03
Selection and Isolation of the Follicles and Retrieval of the COCs
04:31
Long in Vitro Culture of the Oocytes (long IVCO
07:15
Results: Meiotic Progression of Oocytes after long IVCO
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了在发育的早期阶段从卵巢卵泡分离生长的卵母细胞的过程,以及建立体外培养系统,该系统可以支持生长和分化,一次,一次,一次,一个发育阶段。
Tags
In Vitro Culture
Oocytes
Early Antral Follicles
Cattle
Genetic Salvage
Threatened Species
Local Breeds
Ovary Slicing
Follicle Selection
Culture Medium
Cilostamide
Long IVCO Medium
96-well Plate
Incubation Conditions
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