Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

This content is Open Access.

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
 

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Article doi: 10.3791/52408
February 17th, 2015

Summary February 17th, 2015

A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter