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Journal
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Developmental Biology
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牛の初期のアンタル卵胞からの卵母細胞のための体外培養戦略
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Developmental Biology
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JoVE Journal
Developmental Biology
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Please note that all translations are automatically generated.
Click here for the English version.
牛の初期のアンタル卵胞からの卵母細胞のための体外培養戦略
DOI:
10.3791/61625-v
•
09:30 min
•
July 08, 2020
•
Rodrigo G. Barros
,
Valentina Lodde
,
Federica Franciosi
,
Alberto M. Luciano
1
Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety
,
University of Milan
Chapters
00:04
Introduction
00:50
Media Preparation
01:44
Ovary Collection and Processing
03:03
Selection and Isolation of the Follicles and Retrieval of the COCs
04:31
Long in Vitro Culture of the Oocytes (long IVCO
07:15
Results: Meiotic Progression of Oocytes after long IVCO
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
開発初期段階で卵巣卵胞から卵母細胞を分離する手順と、完全に成長した段階までの成長と分化をサポートできるインビトロ培養システムのセットアップについて説明する。
Tags
In Vitro Culture
Oocytes
Early Antral Follicles
Cattle
Genetic Salvage
Threatened Species
Local Breeds
Ovary Slicing
Follicle Selection
Culture Medium
Cilostamide
Long IVCO Medium
96-well Plate
Incubation Conditions
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