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JoVE Journal Bioengineering
Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart
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Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Article DOI: 10.3791/63926-v • 08:20 min • May 19th, 2022
May 19th, 2022 •
1, 1, 2,3, 1
1Department of Physiology and Biophysics, Case Western Reserve University, 2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center, David Geffen School of Medicine, 3UCLA Neurocardiology Research Program of Excellence, UCLA

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Summary
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Established immunochemical methods to measure peptide transmitters in vivo rely on microdialysis or bulk fluid draw to obtain the sample for offline analysis. However, these suffer from spatiotemporal limitations. The present protocol describes the fabrication and application of a capacitive immunoprobe biosensor that overcomes the limitations of the existing techniques.

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Time-resolved In Vivo Measurement Neuropeptide Dynamics Capacitive Immunoprobe Porcine Heart Peptide Transmitter Levels Near-realtime Analysis Perfluoroalkoxy-coated Platinum Wire Gold-plated Connector Pin Dopamine Solution Silver Chloride Disc Electrode Data Acquisition Software Electro-deposition
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