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Journal / Biochemistry / Aislamiento de histone del tejido de la hoja de…
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Biochemistry

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JoVE Journal Biochemistry
Aislamiento de histone del tejido de la hoja de sorgo para la perfilación de espectrometría de masas de arriba hacia abajo de marcadores epigenéticos potenciales
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Aislamiento de histone del tejido de la hoja de sorgo para la perfilación de espectrometría de masas de arriba hacia abajo de marcadores epigenéticos potenciales

Article DOI: 10.3791/61707-v • 07:10 min • March 4th, 2021
March 4th, 2021 •
1, 1, 2, 2, 2, 1, 1, 1, 3, 3, 3, 4,5, 6, 6, 1, 6, 1, 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley

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El protocolo ha sido desarrollado para extraer eficazmente histonas intactas de materiales de hojas de sorgo para la elaboración de perfiles de modificaciones post-traslacionales de piedra histone que pueden servir como marcadores epigenéticos potenciales para ayudar a la ingeniería de cultivos resistentes a la sequía.

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Bioquímica Número 169 sequía epigenética recorte de histona modificaciones post-traslacionales proteómica sorgo espectrometría de masas de arriba hacia abajo
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