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JoVE Journal Biochemistry
Isolatie van histone van Sorghum Leaf Tissue voor Top Down Mass Spectrometry Profiling van potentiële epigenetische markers
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Isolatie van histone van Sorghum Leaf Tissue voor Top Down Mass Spectrometry Profiling van potentiële epigenetische markers

Article DOI: 10.3791/61707-v • 07:10 min • March 4th, 2021
March 4th, 2021 •
1, 1, 2, 2, 2, 1, 1, 1, 3, 3, 3, 4,5, 6, 6, 1, 6, 1, 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley

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Het protocol is ontwikkeld om intacte histonen effectief te extraheren uit sorghumbladmaterialen voor profilering van histon post-translationele modificaties die kunnen dienen als potentiële epigenetische markers om droogtebestendige gewassen te helpen.

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Biochemie droogte epigenetisch histone knipsel post-translationele modificaties proteomics sorghum top down massaspectrometrie
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