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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Article DOI: 10.3791/64534-v • 07:46 min • October 11th, 2022
October 11th, 2022 •
1, 1, 1
1Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University

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Summary
Automatic Translation

The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.

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Three-Dimensional Culture Murine Colonic Crypts Intestinal Stem Cell Function Ex Vivo Organoid Culture Immune-free Environment Knockout Of Claudin-7 Mechanisms Of Stem Cell Function Inflammatory Bowel Disease Colorectal Cancer Isolation Of Colonic Crypts Incision Mouse Model Abdomen Exposure Colon Isolation Adipose Tissue Removal Feces Removal Tissue Washing With PBS Tissue Cutting Into Small Pieces
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