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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
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Cancer Research
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JoVE Journal Cancer Research
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

DOI:
10.3791/64534-v

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07:46 min

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October 11, 2022

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, ,
1Department of Anatomy and Cell Biology, Brody School of Medicine,East Carolina University

Chapters

  • 00:04Introduction
  • 00:51Isolation of Murine Colonic Crypts
  • 03:19Plating the Isolated Murine Colonic Crypts
  • 04:26Harvesting and Embedding the Colonic Organoids
  • 05:50Results: Growth of Claudin‐7 Deficient Colonoids Throughout the 9 Days of Culture
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.

Tags

Keywords: 3D CultureColonic CryptsIntestinal Stem CellsEx VivoOrganoid CultureColonMouse ModelClaudin-7Inflammatory Bowel DiseaseColorectal CancerCrypt IsolationEpithelial DissociationCrypt DissociationCell Culture

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