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Biochemistry

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JoVE Journal Biochemistry
Samtranslationell insättning av membranproteiner i förformade nanodiscs
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Samtranslationell insättning av membranproteiner i förformade nanodiscs

Article DOI: 10.3791/61844-v
November 19th, 2020 •
1, 1, 1, 1
1Institute of Biophysical Chemistry, Goethe University Frankfurt

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November 19th, 2020

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Samtranslationell insättning i förformade nanodiscs gör det möjligt att studera cellfria syntetiserade membranproteiner i definierade lipidmiljöer utan kontakt med tvättmedel. Detta protokoll beskriver beredningen av väsentliga systemkomponenter och de kritiska parametrarna för att förbättra uttryckseffektiviteten och provkvaliteten.

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