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Journal
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Biochemistry
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使用随机微种子基质筛选与 I3C 的蛋白质晶体的衍生
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal
Biochemistry
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Please note that all translations are automatically generated.
Click here for the English version.
使用随机微种子基质筛选与 I3C 的蛋白质晶体的衍生
DOI:
10.3791/61894-v
•
14:04 min
•
January 16, 2021
•
Jia Quyen Truong*
1
,
Stephanie Nguyen*
1,2
,
John B. Bruning
2
,
Keith E. Shearwin
1
School of Biological Sciences
,
The University of Adelaide
,
2
Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences
,
The University of Adelaide
Chapters
00:00
Introduction
01:51
Preparation of 13C Stock
02:24
Addition of I3C to Protein Stock
03:18
Making a Rounded Probe
04:04
Making a Seed Stock
06:33
Setting Up an rMMS Screen
08:08
Data Processing
11:21
Representative Results
13:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种利用微种子在稀疏基质晶波中产生与 I3C(5-氨基-2,4,6-三叶磷酸)衍生的蛋白质晶体的方法。托盘可以使用液体点胶机器人或手动设置。
Tags
Protein Crystal
Derivatization
I3C
Random Microseed Matrix Screening
X-ray Crystallography
Phasing Molecule
Anomalous Signal
Single Wavelength Anomalous Discussion Phasing
Lithium I3C
Protein Crystallization
Structure Solution
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