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Journal / Biochemistry / Derivatization af Protein Krystaller med I3C ved…
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Biochemistry
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JoVE Journal Biochemistry
Derivatization af Protein Krystaller med I3C ved hjælp af Random Microseed Matrix Screening
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Derivatization af Protein Krystaller med I3C ved hjælp af Random Microseed Matrix Screening

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Denne artikel præsenterer en metode til at generere protein krystaller derivatized med I3C (5-amino-2,4,6-triiodoisophthalic syre) ved hjælp af mikroseeding at generere nye krystallisering betingelser i sparsomme matrix skærme. Bakkerne kan sættes op ved hjælp af flydende dispenserrobotter eller i hånden.

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Biokemi såning tilfældig mikroseed matrix screening rMMS protein krystallografi udfasning den magiske trekant I3C
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