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Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
DOI:

09:52 min

February 04, 2021

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Chapters

  • 00:04Introduction
  • 00:44Preparing a Seed Stock of Endothiapepsin Crystals
  • 01:49Morphogram Experiment
  • 03:14Morphogram Analysis
  • 04:06Scaling
  • 05:56Seeded‐Batch Protocol for 20 μm Endothiapepsin Crystals
  • 07:11Results: Representative Endothiapepsin Crystal Growth and Analysis
  • 09:16Conclusion

Summary

Automatic Translation

The aim of this article is to give the viewer a solid understanding of how to transform their small-volume, vapor-diffusion protocol, for growing large, single protein crystals, into a large-volume batch micro-crystallization method for serial crystallography.

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