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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Article DOI: 10.3791/61923-v • 05:58 min • June 6th, 2021
June 6th, 2021 •
1,2, 1,3
1Department of Chemical and Systems Biology, Stanford University School of Medicine, 2Current Address: Department of Biological Sciences, University of Southern California, 3Department of Biochemistry, Stanford University School of Medicine

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Summary
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We describe a method for the preparation and live imaging of undiluted cytoplasmic extracts from Xenopus laevis eggs.

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Xenopus Laevis Egg Extract Preparation Live Imaging Methods Cytoplasmic Dynamics Spatial Pattern Information Cost Effective Easy To Implement FEP Adhesive Tape Glass Slide Roller Applicator FEP Tape Coated Cover Slips Double-sided Sticky Imaging Spacer Glass Beaker Egg-laying Buffer Dejellying Solution Tightly Packed Eggs 0.25x MMR Buffer
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