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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
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Biology
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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

English

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13,531 Views

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10:01 min

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June 27, 2013

DOI:

10.3791/50434-v

DOI:
10.3791/50434-v

•

10:01 min
June 27, 2013

•

8 Views
,
1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Summary

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We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

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Cite this Article

Sharp, J. A., Blower, M. D. Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs. J. Vis. Exp. (76), e50434, doi:10.3791/50434 (2013).

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