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Biology

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

JoVE Journal
Biology

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JoVE Journal Biology

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

DOI:

10.3791/50434-v

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10:01 min

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June 27, 2013

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Judith A. Sharp², Mike D. Blower²

¹Department of Molecular Biology,Massachusetts General Hospital, ²Department of Genetics,Harvard Medical School

Chapters

00:05Title
01:15Generation of X. tropicalis Eggs
03:24Preparation of Extract from X. tropicalis Eggs
06:45Purification of Taxol-stabilized Microtubules from X. tropicalis Extract
08:25Representative X. Tropicalis Extraction Results
09:37Conclusion

Summary

Automatic Translation

We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

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Chapters

Summary

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