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JoVE Journal Neuroscience
Microdisseção do Cérebro de Camundongos em Regiões Funcional e Anatomicamente Diferentes
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Microdisseção do Cérebro de Camundongos em Regiões Funcional e Anatomicamente Diferentes

Article DOI: 10.3791/61941-v • 08:06 min • February 15th, 2021
February 15th, 2021 •
1, 1, 2, 3, 3, 2, 4, 2
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research

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Apresentamos um protocolo prático, passo a passo, rápido para remoção do cérebro do rato e dissecção de regiões discretas de tecido cerebral fresco. A obtenção de regiões cerebrais para análise molecular tornou-se rotina em muitos laboratórios de neurociência. Essas regiões cerebrais são imediatamente congeladas para obter dados transcriômicos de alta qualidade para análise de nível de sistema.

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Neurociência Problema 168 Anatomia cerebral camundongo cérebro dissecção atlas cerebral ensaios moleculares
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