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JoVE Journal Bioengineering
Snabb montering av multigenkonstruktioner med modulär Golden Gate-kloning
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Snabb montering av multigenkonstruktioner med modulär Golden Gate-kloning

Article DOI: 10.3791/61993-v • 08:31 min • February 5th, 2021
February 5th, 2021 •
*1, *1, 1
1Department of Biological Sciences, University at Buffalo, State University of New York
* These authors contributed equally

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Målet med detta protokoll är att tillhandahålla en detaljerad steg-för-steg-guide för montering av multigenkonstruktioner med hjälp av det modulära kloningssystemet baserat på Golden Gate-kloning. Det ger också rekommendationer om kritiska steg för att säkerställa optimal montering baserat på våra erfarenheter.

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