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JoVE Journal Bioengineering
Assemblaggio rapido di costrutti multi-gene utilizzando la clonazione modulare del Golden Gate
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Assemblaggio rapido di costrutti multi-gene utilizzando la clonazione modulare del Golden Gate

Article DOI: 10.3791/61993-v • 08:31 min • February 5th, 2021
February 5th, 2021 •
*1, *1, 1
1Department of Biological Sciences, University at Buffalo, State University of New York
* These authors contributed equally

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L'obiettivo di questo protocollo è fornire una guida dettagliata e dettagliata per assemblare costrutti multi-genico utilizzando il sistema di clonazione modulare basato sulla clonazione di Golden Gate. Fornisce anche raccomandazioni sui passaggi critici per garantire un assemblaggio ottimale in base alle nostre esperienze.

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Bioingegneria Numero 168 MoClo Saccharomyces cerevisiae,ingegneria metabolica espressione eterologo CRISPR genome editing
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