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从鸡胚胎的直膜到研究棒到锥细胞相互作用的锥形丰富文化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
Please note that all translations are automatically generated.
Click here for the English version.
从鸡胚胎的直膜到研究棒到锥细胞相互作用的锥形丰富文化
DOI:
10.3791/61998-v
•
08:04 min
•
March 20, 2021
•
Géraldine Millet-Puel
,
Myriam Pinault
,
Marie Cordonnier
,
Valérie Fontaine
,
José-Alain Sahel
,
Thierry Léveillard
1
Department of Genetics – Sorbonne Université, INSERM, CNRS
,
Institut de la Vision
Chapters
00:04
Introduction
00:58
Recovery of the Chicken Embryos
02:11
Dissection of the Retinas
02:50
Preparation of Retinal Cell Suspension
03:40
Retinal Cell Seeding
05:16
Results: Epithelium-Derived Cone Viability Factor (EdCVF), Clone 0073-09-37
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种从鸡胚胎的网膜中获取锥体光感受器的初级培养物的方法,并将其用于高含量筛选。
Tags
Cone Receptors
Chicken Embryo
Rod-cone Interactions
Retinal Degeneration
Cone-enriched Culture
Primary Cell Culture
Hamburger And Hamilton Stages
Carbon Dioxide-independent Medium
Ringer’s Medium
Trypsin
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