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Definizione del programma di traduzione dell'mRNA materno durante la maturazione in vitro utilizzando un saggio single oocyte reporter
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Definizione del programma di traduzione dell'mRNA materno durante la maturazione in vitro utilizzando un saggio single oocyte reporter

Article DOI: 10.3791/62041-v • 08:00 min • June 16th, 2021
June 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2
1Center for Reproductive Sciences, University of California at San Francisco, 2Department of Obstetrics Gynecology and Reproductive Sciences, University of California at San Francisco, 3Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS, Sorbonne Université

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Questo protocollo descrive un saggio di reporter per studiare la regolazione della traduzione dell'mRNA in singoli ovociti durante la maturazione in vitro.

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Biologia dello sviluppo Numero 172 Ovocita mRNA traduzione maturazione meiotica microscopia time-lapse saggio reporter micro-iniezione
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