Journal
/
/
Definiera programmet för moderns mRNA-översättning under in vitro-mognad med hjälp av en enda Oocyte Reporter-analys
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
DOI:

08:00 min

June 16, 2021

, , ,

Chapters

  • 00:04Introduction
  • 00:38Aspiration of Denuded Oocyte and its Microinjection
  • 03:18Time-lapse Microscopy and Analysis of Ypet-3 UTR Translation
  • 06:00Results: Statistical Analysis of the Expression for Reporter-YFP and mCherry in Prophase I and Maturing Oocytes
  • 07:19Conclusion

Summary

Automatic Translation

Detta protokoll beskriver en reporter analys att studera regleringen av mRNA översättning i enstaka äggceller under in vitro mognad.

Related Videos

Read Article