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Definindo o Programa de Tradução materna mRNA durante a maturação in vitro usando um ensaio de repórter oócito único
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
DOI:

08:00 min

June 16, 2021

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Chapters

  • 00:04Introduction
  • 00:38Aspiration of Denuded Oocyte and its Microinjection
  • 03:18Time-lapse Microscopy and Analysis of Ypet-3 UTR Translation
  • 06:00Results: Statistical Analysis of the Expression for Reporter-YFP and mCherry in Prophase I and Maturing Oocytes
  • 07:19Conclusion

Summary

Automatic Translation

Este protocolo descreve um ensaio repórter para estudar a regulação da tradução de mRNA em oócitos únicos durante a maturação in vitro.

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