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Skøn over indholdet af plantebiomasse lignin ved hjælp af thioglykolsyre (TGA)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)
DOI:

09:25 min

July 24, 2021

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Chapters

  • 00:00Introduction
  • 01:15Protocol: Phase I- Preparation of Plant Samples
  • 02:34Protocol: Phase II- Preparation of Alcohol Insoluble Material (Water and Methanol Washes)
  • 03:46Protocol: Phase III- Treatment of Pellet with TGA and Acid to Precipitate Lignin
  • 06:22Protocol: Phase IV- Results (Ab280mm Reading)
  • 07:01Representative Results
  • 08:38Conclusion

Summary

Automatic Translation

Her præsenterer vi en modificeret TGA-metode til vurdering af ligninindholdet i urteagtig plantebiomasse. Denne metode anslår ligninindholdet ved at danne specifikke tredistre obligationer med lignin og giver en fordel i forhold til Klason-metoden, da den kræver en relativt lille stikprøve til skøn over ligninindholdet.

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