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JoVE Journal Neuroscience
Analyse van vertaling in de zich ontwikkelende muishersenen met behulp van polysoomprofilering
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Analyse van vertaling in de zich ontwikkelende muishersenen met behulp van polysoomprofilering

Article DOI: 10.3791/62088-v • 08:38 min • May 22nd, 2021
May 22nd, 2021 •
1,3, 2,3, 1,2,3
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine, University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary

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De ontwikkeling van het zoogdierbrein vereist een goede controle van genexpressie op het niveau van vertaling. Hier beschrijven we een polysoomprofileringssysteem met een eenvoudig te assembleren sucrose gradiëntvormings- en fractioneringsplatform om de translationele status van mRNAs in de zich ontwikkelende hersenen te beoordelen.

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Neurowetenschappen Nummer 171
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