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评估穆林伤口的生物膜分散
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Biofilm Dispersal in Murine Wounds
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评估穆林伤口的生物膜分散

DOI:
10.3791/62136-v

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12:18 min

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August 07, 2021

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, ,
1Department of Surgery,Texas Tech University Health Sciences Center, 2Immunology and Molecular Microbiology,Texas Tech University Health Sciences Center, 3TTUHSC Surgery Burn Center of Research Excellence,Texas Tech University Health Sciences Center

Chapters

  • 00:00Introduction
  • 01:24Dorsal Full Thickness Excision Surgery
  • 03:25In vivo Treatment and Assessment of Dispersal
  • 07:04Ex vivo Assessment of Dispersal
  • 09:12Results: Enzymatic Dispersal Leads to Bacterial Dissemination in the Host
  • 10:55Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了 前活体 和 体内 方法，以评估细菌分散从伤口感染在小鼠。该协议可用于测试局部抗菌和抗生物膜疗法的疗效，或评估不同细菌菌株或物种的分散能力。

Tags

BiofilmBiofilm DispersalMurine Wound ModelChronic InfectionBacterial InfectionDispersal AgentAnesthesiaWound ExcisionWound InfectionCFUBacterial Inoculation

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